Electrochemical studies of lateral flow assay test results for procalcitonin detection

In this study, the lateral flow assay (LFA) has been developed for detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need complicated instrumentations and technical expertise. For development assay, gold nanoparticles (AuNP) their conjugates with antibodies to model antigen PCT are assessed. Polyclonal antibody (pAb) labelled obtain AuNP-pAb complex monoclonal (mAb) have dropped at test zone. This is placed over conjugate line LFA strip. absence or presence other biomarkers, remained colourless, which revealed specificity towards among pool various analytes. Herein, observations made through two different platforms quantitative qualitative analysis biomarker. The performed on basis appearance red color in band, while analysis, novel approach adopted. nitrocellulose membrane (paper strip) cut out from strip used electrochemical studies under similar solution conditions. Different paper strips presented cyclic voltammograms (CV) that could be correlated varying concentrations captured limit using was determined 2 ng/ml 1 ng/ml. response CV technique sensitivity value 0.695 mA